期刊论文

中文

中国病理生理杂志

1000-4718

2005

21

6

1041-1045

10.3321/j.issn:1000-4718.2005.06.001

国家自然科学基金资助项目（No.30171084;No.30470719）； 湖南省自然科学基金资助项目（No.01JJY2145）；

本校为第一机构

目的:研究细胞内胆固醇流出对单核细胞源性泡沫细胞凋亡的影响. 方法: 用50 mg/L ox-LDL与RAW264.7细胞共同孵育建立泡沫细胞模型,采用流式细胞仪检测细胞凋亡率,油红O观察细胞内脂滴的形成情况,用[3H]-胆固醇标记法检测细胞内胆固醇流出率,HPLC检测细胞内胆固醇含量. 结果: 用50 mg/L ox-LDL与RAW264.7细胞共同孵育48 h后,细胞内形成大量脂滴,细胞内胆固醇酯含量由(6.8±3.6)mg/g升至(101.7±4.5)mg/g,细胞凋亡率由8.14%升至42.6%(P

AIM: To determine the effect of promoting cellular cholesterol efflux on the apoptosis of foam cells derived from monocytes. METHODS: RAW264.7 cells were incubated with 50 mg/L ox-LDL as a foam cell mode. The apoptosis rate of RAW264.7 cells was assayed by flow cytometry. Cellular lipid droplet was assayed by oil red staining. The rate of cellular cholesterol efflux was assayed with [~3H] label cholesterol, and the content of cellular cholesterol were assayed by high performance liquid chromatography. RESULTS: After incubation with 50 mg/L ox-L...