期刊论文

中文

中国病理生理杂志

1000-4718

2005

21

4

690-695

10.3321/j.issn:1000-4718.2005.04.014

湖南省教育厅科研基金资助项目 （No .0 1C191）；

本校为第一机构

目的:研究ADRP与As发生和发展的关系.方法:使用80 mg/L Ox-LDL和/或1 mmol/L反义ADRP寡核苷酸与小鼠腹腔巨噬细胞共孵育72 h.测定细胞内胆固醇酯;油红O染色显示细胞内中性脂质;RT-PCR和Western blotting观察反义寡核苷酸对ADRP表达的影响.高胆固醇饲料喂养新西兰白兔12周,复制As模型.测定血清总胆固醇、低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、甘油三酯及动脉壁胆固醇;苏丹Ⅳ染色显示主动脉病变面积;HE染色观察主动脉及肝脏的病变;免疫组织化学方法显示ADRP在主动脉病变区及肝脏中的表达.结果:反义ADRP寡核苷酸使细胞内胆固醇酯由(46.6±3.4)mg/g protein下降到(19.9±1.9)mg/g protein,细胞内...

AIM: To investigate the relationship betw een ADRP and the development of atherosclerosis. METHODS: Antisense oligodeoxynucleotide of mouse ADRP was constr ucted. The mouse peritoneum macrophages were cultured with Ox-LDL or Ox-LDL plus the antisense fragment. The cellular cholesterol was measured and the expressio n of ADRP was observed with RT-PCR and western blotting. New Zealand white rabbi ts were fed with high cholesterol chow for 12 weeks. The levels of serum lipid a nd cholesterol content of aortic wall were investigated. The areas of f...