期刊论文

Quantitative Proteomic Study for Human Colon Cancer Tissue by Laser Capture Microdissection

中文

基因组学与应用生物学

1674-568X

2017

36

8

3358-3367

10.13417/j.gab.036.003358

本研究由国家重点基础研究发展计划（973计划）（2011CB910704 2014CBA02004）、国家自然科学基金（81272971 81202129 81372516）和湖南省教育厅资助科研项目（16C1409）共同资助

本校为其他机构

为分析结肠上皮癌变进程中的差异表达蛋白质,筛选结肠癌诊断候选标志物,先采用激光捕获显微切割技术(LCM)纯化人正常结肠组织和结肠癌组织,再采用同位素标记对蛋白质进行相对和绝对定量(iTRAQ),然后结合强阳离子柱分离和反相液质联用(2D-LC-MS/MS)鉴定差异表达蛋白质。试验共鉴定了1 042个非冗余蛋白,筛选出与人结肠癌变相关的差异蛋白162个,其中在结肠癌中表达上调的蛋白质68个,表达下调的蛋白质94个,功能分析表明,这些差异蛋白质涉及内质网蛋白质加工、糖酵解/糖原异生、黏着斑细胞连接、调节微丝组装和细胞外基质受体相互作用等分子功能。进一步采用Western blotting及免疫组织化学技术(IHC)...

In order to analyze differentially expressed proteins in carcinogenesis of colonic epithelium and screen candidate markers for colon cancer diagnosis, human normal colon and colon cancer tissues were purified by laser capture microdissection （LCM）. Proteins were quantified by isobaric tags for relative and absolute quantitation （iTRAQ）, then the differentially expressed proteins were identified by strong cation column separation and reverse phase liquid chromatography （2D-LC-MS/MS）. In the research, 1 042 non-redundant proteins were ident...