期刊论文

中文

生命科学研究

1007-7847

2022

26

3

200-207

湖南省自然科学基金资助项目（2020JJ5479）； 湖南省教育厅一般项目（19C1604）； 湖南省大学生创新训练项目（S202010555135）；

本校为第一机构

黑腹果蝇作为经典遗传学和分子遗传学模式生物,已经成为研究基因调控器官发育以及各种疾病发生的一个强有力的模型。为了探究Mlp84B在果蝇体内的表达定位及互作蛋白质,首先将含启动子元件的果蝇Mlp84B基因组融合GFP序列克隆至pUAST表达载体中,利用胚胎显微注射技术将构建好的重组质粒注射到果蝇早期受精卵;其次通过杂交获得红眼果蝇,经单果蝇建系、平衡子定位后获得稳定遗传的转基因果蝇;最后通过在果蝇体内检测到GFP的表达,同时经qRT-PCR检测到Mlp84B的表达水平上升,证实转基因果蝇构建成功。GFP定位结果显示,内源Mlp84B在果蝇肌肉系统表达,并且Mlp84B与Act57B在果蝇体内相互作用。实验结果表明...

Drosophila melanogaster,as a model organism of both classical and molecular genetics,has become a powerful model for studying gene regulation of organ development and the occurrence of various diseases.In order to investigate the expression and localization of Mlp84B,and its interactive proteins in Drosophila,firstly,the Drosophila Mlp84B-GFP fusion sequence containing a promoter was cloned into pUAST expression vector,and the constructed recombinant plasmid was injected into the early fertilized eggs of Drosophila using embryo microinjection technique.Secondly,red-eyed Drosophila was obtained...