期刊论文

中文

现代肿瘤医学

1672-4992

2019

27

7

1135-1140

10.3969/j.issn.1672-4992.2019.07.009

湖南省教育厅重点课题(编号:17A190) 衡阳市科技局项目(编号:S2018F9031025334).

本校为第一机构

目的:探讨MDA-MB-231、MCF-7乳腺癌细胞条件培养基对hFOB1.19成骨细胞的增殖抑制作用与Notch信号通路的相关性。方法:MTT法检测MDA-MB-231、MCF-7乳腺癌细胞条件培养基对hFOB1.19细胞的增殖抑制效果;Western Blot与qRT-PCR分别检测hFOB1.19细胞的Notch1、Jagged1、Hes1蛋白与mRNA的表达。结果:MDA-MB-231、MCF-7条件培养基抑制hFOB1.19细胞增殖,DAPT（Notch阻断剂）可明显降低MDA-MB-231、MCF-7条件培养基对hFOB1.19细胞增殖的抑制作用。MDA-MB-231、MCF-7条件培养基上调hFOB1.19细胞Notch1、Jagged1、Hes1的mRNA和蛋白的表达,并随着条件培养基浓度的增加,以上指标的表达呈先上升而后下降的趋势...

Objective:To investigate the relationship between the inhibitory effect of MDA-MB-231 and MCF-7 breast cancer cell conditioned medium on the proliferation of hFOB1.19 osteoblast and the Notch signal pathway.Methods:MTT assay was used to detect the inhibition of hFOB1.19 osteoblast proliferation.The protein and mRNA expression of Notch1,Jagged1 and Hes1 in hFOB1.19 osteoblast were detected by Western Blot and qRT-PCR respectively.Results:The MDA-MB-231 and MCF-7 conditioned medium inhibited the proliferation of hFOB1.19 cells.DAPT(Notch blocker) could significantly reduce the effect of the cond...