期刊论文

The interaction of EGFR mutation with smoking and correlation between EGFR mutation and miR-221 expression in NSCLC

中文

现代肿瘤医学

1672-4992

2019

27

8

1351-1355

10.3969/j.issn.1672-4992.2019.08.015

湖南省教育厅高校科研项目(编号:15C1199) 湖南省自然科学基金项目(编号:2018JJ2342).

本校为第一机构

目的:分析NSCLC EGFR突变与吸烟等因素的交互作用,及其与miR-221表达的相关性,为肺癌分子机制研究提供线索和依据.方法:采用高分辨熔解曲线法(high resolution melting,HRM)进行EGFR突变检测,以EGFR突变阳性为病例组,野生型为对照组,logistic回归分析EGFR突变与吸烟等因素在肺癌中的交互作用,Spearman分析EGFR突变与miR-221表达的相关性.结果:吸烟者相对于不吸烟者,EGFR突变差异具有统计学意义(P =0.034),不吸烟者EGFR突变率较高.性别、年龄、饮酒、组织学类型、肿瘤直径、TNM分期、分化程度、肿瘤家族史以及心脑血管疾病史与EGFR突变无统计学关系(P＞0.05);NSCLC EGFR突变与吸烟、饮酒、肿瘤家...

Objective:To detect the interaction of epidermal growth factor receptor(EGFR) mutations with smoking and other factors in non-small cell lung cancer(NSCLC).And find out the correlation between miR-221 expression and EGFR mutations to provide clue and basis for studying the potential molecular mechanism of lung cancer.Methods:We took the EGFR mutation as the case group and the wild type for the control group to analyse the interaction of EGFR mutation with smoking & other factors by logistic regression.EGFR mutant was detected by high resolution melting(HRM).The correlation between miR-221 expr...