Construct Program of the Key Discipline in Hunan Province, Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control [Hunan Provincial Science & Technology Department document] [2014-5]; Construct Program of the Key Discipline in Hunan Province, Hunan Provincial Key Laboratory for Special Pathogen Prevention and Control [Hunan Provincial Education Department document] [2012-312]; Key Project of Hunan Provincial Education DepartmentHunan Provincial Education Department [11A103]; Research Project of Hunan Provincial Science & Technology Department [2013TT2021]
机构署名：
本校为第一且通讯机构
院系归属：
医学院
摘要：
This study investigated whether Helicobacter pylori could activate the nucleotide-binding oligomerization domain-like receptor (NLR) family, pyrin domain-containing 3 (NLRP3) inflammasome in human macrophages and the involvement of reactive oxygen species (ROS) in inflammasome activation. Phorbol-12-myristate-13-acetate (PMA)-differentiated human acute monocytic leukemia cell line THP-1 was infected with H. pylori. The levels of pro-inflammatory cytokines interleukin (IL)-1β and IL-18 in supernatant were measured by ELISA. Intracellular ROS le...
