期刊论文

中文

卫生研究

1000-8020

2017

46

2

318-323

10.19813/j.cnki.weishengyanjiu.2017.02.027

本校为第一机构

目的建立固相萃取-高效液相色谱法测定多种类食品中靛蓝、亮蓝的检测方法。方法采用固相萃取技术,用乙腈水溶液提取样品中色素并经WAX 小柱净化,以甲醇-0. 02 mol /L 乙酸铵溶液作为流动相梯度洗脱,经Waters Symmetry C18(5 μm,4. 6 mm × 250 mm)色谱柱分离,运用高效液相色谱-二极管阵列检测器(HPLC-PDA)检测。结果该方法靛蓝、亮蓝在0. 50 ~ 20. 00 μg /mL 范围内有较好的线性关系,均r > 0. 999; 靛蓝和亮蓝的方法检出限分别为0. 04和0. 02 mg /kg; 样品加标平均回收率为81. 8% ~ 101. 1%,相对标准偏差为2. 1% ~ 4. 9%(n = 6)。结论该方法具有较高的选择性和灵敏度,回收率和重现性良好,可用...

OBJECTIVE: To develop and validate a solid phase extraction-high performance liquid chromatographic( SPE-HPLC) method for the simultaneous determination of indigo and brilliant blue in different types of food products. METHODS: The artificial colors in food products were extracted by acetonitrile / water and purified by WAX SPE cartridges, The separation was achieved using a Waters Symmetry C_(18)( 5 mum, 4. 6 mm x 250 mm) column and a binary gradient mobile phase of methanol and 0. 02 mol/L ammonium acetate solution, detected by HPLC-PDA. RESULTS: The validated analytical method showed that t...