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Regulation of the Golgi apparatus via GOLPH3-mediated new selective autophagy

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成果类型:
期刊论文
作者:
Lu, Li-qun;Tang, Ming-zhu;Qi, Zhi-hao;Huang, Shi-fang;He, Yong-qi;...
通讯作者:
Li, Lan-fang;Chen, Lin-xi
作者机构:
[Huang, Shi-fang; Chen, Lin-xi; Qi, Zhi-hao; He, Yong-qi; Li, Lan-fang; Tang, Ming-zhu; Li, LF; Chen, LX; Lu, Li-qun; Li, Dian-ke] Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Learning Key Lab Pharmacoprote, Hengyang 421001, Peoples R China.
通讯机构:
[Li, LF; Chen, LX] U
Univ South China, Inst Pharm & Pharmacol, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Learning Key Lab Pharmacoprote, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
cell protein;Golgi phosphoprotein 3;unclassified drug;GOLPH3 protein, human;MAP1LC3B protein, human;membrane protein;microtubule associated protein;animal cell;Article;autophagosome;autophagy (cellular);cardiac muscle cell;cell stress;cell survival;controlled study;Golgi complex;H9c2(2-1) cell line;HEK293T cell line;homeostasis;human;human cell;HUVEC cell line;immunoprecipitation;light chain;nonhuman;rat;transmission electron microscopy;vascular smooth muscle cell;animal;autophagy;cell line;gene knockdown;genetics;Golgi complex;HEK293 cell line;metabolism;physiology;umbilical vein endothelial cell;Animals;Autophagy;Cell Line;Cell Survival;Gene Knockdown Techniques;Golgi Apparatus;HEK293 Cells;Human Umbilical Vein Endothelial Cells;Humans;Immunoprecipitation;Membrane Proteins;Microscopy, Electron, Transmission;Microtubule-Associated Proteins;Myocytes, Cardiac;Rats
期刊:
Life Sciences
ISSN:
0024-3205
年:
2020
卷:
253
期:
1
页码:
117700
基金类别:
This work was supported by the grants from the National Natural Science Foundation of China ( 81470434 , 81670265 ).
机构署名:
本校为第一且通讯机构
院系归属:
药学与生物科学学院
摘要:
Aims: Although previous studies elaborated that selective autophagy was involved in quality control of some organelles, including nucleus, mitochondria, the endoplasmic reticulum and peroxisomes, it remained unclear whether the selective autophagy of the Golgi apparatus (Golgiphagy) existed or not. Main methods: In this study, H9c2 cells, HUVECs, HA-VSMCs and HEK293T cells were treated with autophagy inducers, Golgi stress inducers and cardiomyocytes hypertrophy stimulators. The Golgiphagy was evaluated by analysing the co-localization of Golgi...

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