期刊论文

Wu YiMou

英文

中国科学:生命科学(英文版)

1674-7305

2011

54

11

1048-1054

10.1007/s11427-011-4243-1

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30970165, 81102230]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [09JJ3059]; Team Project for the Technology Innovation of Higher Education of Hunan Province, China

本校为第一且通讯机构

The inclusion membrane proteins play potentially important roles in chlamydial biology and pathogenesis. Here we localized and characterized the hypothetical protein CT440 in Chlamydia trachomatis-infected cells. The open reading frame (ORF) encoding the CT440 protein from the C. trachomatis serovar D genome was cloned into the prokaryotic expression vector pGEX-6p and expressed as a glutathione-S-transferase (GST) fusion protein in E. coli XL1-Blue. The CT440 fusion protein was used to immunize mice to raise antigen-specific antibody. After verification by Western blot and immunofluorescence ...