期刊论文

Wu Yi Mou

英文

生物医学与环境科学：英文版

0895-3988

2013

26

3

169-175

10.3967/0895-3988.2013.03.003

*This research was supported by grants from the National Natural Science Foundation of China (30970165 and 81102230), Hunan Provincial Natural Science Foundation of China (09JJ3059) and Team Project for the Technology Innovation of Higher Education of Hunan province, China, 2010. △LI Zhong Yu and HUANG Qiu Lin contributed equally to this work. #Correspondence should be addressed to WU Yi Mou, Tel: 86 ?734 ?8282907. E ?mail: lzhy1023@hotmail.com Biographical note of the first authors: LI Zhong Yu, female, born in 1972, Professor and Ph.D, majoring in pathogenesis and diagnosis of C. trachomatis; HUANG Qiu Lin, male, born in 1973, Chief Physician and Ph.D, majoring in pathogenesis of hepatocellular carcinoma. Received: December 28, 2011; Accepted: December 18, 2012

本校为第一且通讯机构

Objective To prepare antibodies against pORF5 plasmid protein of Chlamydia trachomatis and develop double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) for the detection of genital C. trachomatis infections. Methods The pORF5 protein was expressed in Escherichia coli and used to immunize BALB/c mice and New Zealand rabbits to produce monoclonal antibodies (mAbs) and polyclonal antibody (pAb) for DAS-ELISAs. Clinical samples from 186 urogenital infection patients (groups I) and 62 healthy donors (groups II) were detected in p...

对 Chlamydia trachomatis 的 pORF5 plasmid 蛋白质准备抗体并且开发双抗体三明治的目的为生殖器的 C 的察觉的连接酶的 immunosorbent 试金(DAS-ELISAs ) 。trachomatis 感染。pORF5 蛋白质在 Escherichia coli 被表示并且过去常使 BALB/c 老鼠和新西兰兔子免疫为 DAS-ELISAs 生产 monoclonal 抗体(mAbs ) 和 polyclonal 抗体(pAb ) 的方法。从 186 个尿生殖的感染病人的临床的样品(组我) 并且 62 个健康施主(组 II ) 被在这研究并且由 IDEIA PCE 商业 ELISA 开发的 DAS-ELISAs 在平行检测。二个 hybridoma 房间衬里的结果，命名 2H4 和 4E6，稳定地 secreting 对 pORF5 的特定的 mAbs 被获得...