期刊论文

Wan Kang Lin

英文

生物医学与环境科学：英文版

0895-3988

2013

26

3

190-200

10.3967/0895-3988.2013.03.006

*This research was supported by the 12th Five ?Year Major National Science and Technology Projects of China (No.2011ZX10004 ?001) and Natural Science Foundation of China (31100105). +LIU Zhi Yun, HAO Qin, and HOU Xue Xia contributed equally to this study. #Correspondence should be addressed to: WAN Kang Lin. Tel: 86 ?10 ?58900776. Fax: 86 ?10 ?58900779. E ?mail: wangkanglin@icdc.cn Biographical note of the first authors: LIU Zhi Yun, female, born in 1980, chief examiner; HAO Qin, female, born in 1975, Ph.D, majoring in Lyme disease; HOU Xue Xia, female, born in 1968, associate technician, majoring in Lyme disease. Received: November 15, 2011; Accepted: May 4, 2011

本校为其他机构

Objective To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure. Methods FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS-PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel-Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accura...

目的将为 Borrelia afzelii 在中国引起的 Lyme 疾病的诊断学习西方的污点的技术并且由运作的过程建立标准标准。方法 FP1，它是 B 的代表性的紧张。在中国的 afzelii，被 SDS 页，电镀物品转移和 immunoblotting 试金分析。FP1 的蛋白质乐队的分子的重量被胶化职业人员分析软件分析。在用 451 件浆液样品(有 Lyme 疾病和 292 控制的 159 个病人) 的研究，所有观察乐队被记录。诊断测试被使用 ROC 曲线和 Youden 索引建立的 WB 的精确性。为 Lyme 疾病的积极诊断的结果标准在 IgG 测试和 P83/100 的至少一个乐队作为 P83/100 ， P58 ， P39 ， OspB ， OspA ， P30 ， P28 ， OspC ， P17 ，和 ...