期刊论文

Wu Yi Mou

英文

生物医学与环境科学：英文版

0895-3988

2012

25

6

690-696

10.3967/0895-3988.2012.06.011

*This project was supported by grants from the National Natural Science Foundation of China (Grant No. 30901352), Innovative Research Team in University of Hunan Province (Number: [2008] 51), Hunan Provincial Innovation Foundation for Postgraduate and Hunan Provincial Training and Innovation Base for Post-graduate. #Correspondence should be addressed to WU Yi Mou. Tel: 86-734-8282907. Fax: 86-734-8282907. E-mail: yimouwu@sina.com Biographical note of the first author: ZHOU Zhou, female, born in 1980, Ph. D candidate, majoring in pathogenesis and diagnosis of Chlamydophila pneumoniae. Received: August 21, 2011; Accepted: May 31, 2012

本校为第一且通讯机构

Objective: This paper aims to develop a monoclonal antibodies (MAbs)- based ELISA for detecting Chlamydophila pneumoniae (C. pneumoniae) antigens in humans with the variable domains (VD) 2 and 3 of the major outer membrane protein (MOMP VD2-VD3 ) and to assess its sensitivity and specificity by comparing with a widely used MAb that is able to recognize the elementary bodies of C. pneumoniae. Methods: MOMP VD2-VD3 were overexpressed in Escherichia coli and purified by affinity chromatography. Mice were immunized with the recombinant antigen, and...

这份报纸试图开发 monoclonal 的目的抗体(MAbs )- 为检测 Chlamydophila pneumoniae 的基于的 ELISA (C。pneumoniae ) 在有可变领域(VD ) 的人的抗原 2 专业和 3 外部膜蛋白质(MOMPVD2-VD3 ) 并且认出 C 的基本身体由与对有能力的广泛地使用的 MAb 作比较估计它的敏感和特性。pneumoniae。方法 MOMPVD2-VD3 是在 Escherichia coli 的 overexpressed 并且由亲密关系层析净化了。老鼠与 recombinant 抗原被使免疫，并且 hybridomas secreting MAbs 被屏蔽。三稳定的 hybridomas 克隆被选择并且称为 5D6， 7G3，和 8C9。基于 MAbs 的 ELISA 从组与很多件人的喉咙拖把样品为种类特定的识别被细看我...