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Caveolae and caveolin-1 mediate endocytosis and transcytosis of oxidized low density lipoprotein in endothelial cells

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成果类型:
期刊论文
作者:
Sun, Shao-wei;Zu, Xu-yu;Tuo, Qin-hui;Chen, Lin-xi;Lei, Xiao-yong;...
通讯作者:
Tang, Chao-ke
作者机构:
[Tang, Chao-ke; Liao, Duan-fang; Sun, Shao-wei] Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
[Chen, Lin-xi; Liao, Duan-fang; Lei, Xiao-yong; Tuo, Qin-hui; Sun, Shao-wei; Zu, Xu-yu] Univ S China, Inst Pharm & Pharmacol, Prov Key Lab Pharmacoprotom, Hengyang 421001, Peoples R China.
[Liao, Duan-fang] Hunan Univ Chinese Med, Dept Tradit Chinese Diagnost, Sch Pharm, Changsha 410208, Hunan, Peoples R China.
[Li, Kai] Suzhou Univ, Mol Med Ctr, Suzhou 215004, Peoples R China.
[Li, Kai] Suzhou Univ, Affiliated Hosp 2, Suzhou 215004, Peoples R China.
通讯机构:
[Tang, Chao-ke] U
Univ S China, Life Sci Res Ctr, Key Lab Atherosclerol Hunan Prov, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
caveolae;caveolin-1;oxidized low density lipoprotein;atherosclerosis;transcytosis;human umbilical vein endothelial cells
关键词(中文):
氧化低密度脂蛋白;血管内皮细胞;小窝;内吞作用;氧化型低密度脂蛋白;人脐静脉内皮细胞;荧光分光光度计;高效液相色谱法
期刊:
中国药理学报
ISSN:
1671-4083
年:
2010
卷:
31
期:
10
页码:
1336-1342
基金类别:
Acknowledgements The authors gratefully acknowledge financial support from the National Natural Sciences Foundation of China (No 30971170) and the National Major Basic Research Program of China (973 Program) (No 2006CB503808).
机构署名:
本校为第一且通讯机构
院系归属:
国防科学技术学院
医学院
药学与生物科学学院
摘要:
Aim: To explore the mechanisms involved in ox-LDL transcytosis across endothelial cells and the role of caveolae in this process. Methods: An in vitro model was established to investigate the passage of oxidized low density lipoprotein (ox-LDL) through a tight monolayer of human umbilical vein endothelial cells (HUVEC) cultured on a collagen-coated filter. Passage of DiI-labeled ox-LDL through the monolayer was measured using a fluorescence spectrophotometer. The uptake and efflux of ox-LDL by HUVEC were determined using fluorescence microscopy...

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