Project supported by grants from Major State Basic ResearchDevelopment Program of China
No 2000056905 and grants fromthe Special Funds for Department of Science and Technology ofHu-nan Province
No 01SSY1003.
机构署名：
本校为第一且通讯机构
院系归属：
医学院
摘要：
AIM: To study the effect of oxidized low density lipoprotein (ox-LDL) on ATP binding cassette transporter A1 (ABCA1) in THP-1 macrophages. METHODS: After exposing the cultured THP-1 macrophages to ox-LDL for different periods, cholesterol efflux was determined by FJ-2107P type liquid scintillator. ABCA1 mRNA and protein level were determined by reverse trancriptase-polymerase chain reaction (RT-PCR) and Western blot, respectively. The cholesterol level in THP-1 macrophage foam cells was detected by high performance liquid chromatography. RESULTS: ox-LDL elevated ABCA1 in both protein and mRNA ...
