期刊论文

Lin, Ying-Wu

英文

DALTON TRANSACTIONS

1477-9226

2017

46

34

11230-11238

10.1039/c7dt02302b

It is a pleasure to acknowledge Prof. S. G. Sligar and Prof. Y. Lu of the University of Illinois at Urbana-Champaign, for the kind gift of the sperm whale Mb gene. We also thank Dr Ji-Hu Su of the University of Science and Technology of China, for helpful discussions regarding EPR data. X-ray diffraction data were collected at Shanghai Synchrotron Radiation Facility (SSRF), China. This work was supported by the National Natural Science Foundation of China (31370812), the Hunan Provincial Natural Science Foundation (2015JJ1012) and the Scientific Research Fund of Hunan Provincial Education Department (15A158).

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The rational design of metalloenzymes provides advantages not only for illustrating the structure and function relationship of native enzymes, but also for creating functional artificial enzymes comparable to native enzymes. Dye-decolorizing peroxidases (DyPs) are a new family of heme peroxidases and have received much attention recently. Inspired by the structural features of native DyPs with multiple Tyr and Trp residues, we herein aimed to design functional artificial DyPs using myoglobin (Mb), an O2 carrier as a protein scaffold, by further...