期刊论文

The protective effect of microRNA-126 mimic on tumor necrosis factor-α induced vascular endothelial cell injury

中文

中华高血压杂志

1673-7245

2020

28

9

862-867

10.16439/j.cnki.1673-7245.2020.09.016

本校为第一机构

目的探究微小RNA(miR)-126模拟物在肿瘤坏死因子α(TNF-α)诱导的血管内皮细胞损伤中的作用及可能机制。方法体外培养人脐静脉内皮细胞株ECV304,随机进行如下处理:无特殊处理(对照组);加100 μg/L TNF-α(TNF-α组);转染Lipofectamine 2000+100 μg/L TNF-α(miR-126阴性组);转染miR-126模拟物+100 μg/L TNF-α(miR-126模拟物组),每组设置6个重复样品。实时荧光定量聚合酶链反应(qRT-PCR)法检测细胞中miR-126表达情况;MTT法检测细胞增殖活性;酶联免疫吸附试验法检测细胞中白细胞介素(IL)6、IL-1β水平;流式细胞术检测细胞凋亡情况;免疫印迹法检测细胞中内皮细胞黏附分子1(VCAM-1)、细胞间黏附分...

Objective To explore the role of microRNA(miR)-126 in the injury of vascular endothelial cells induced by tumor necrosis factor-α (TNF-α) and its possible mechanism. Methods ECV304 cells were cultured in vitro and randomly divided into four groups: control group(did nothing), TNF-α group(added 100 μg/L TNF-α), miR-126 negative group(transfected lipofectamine 2000+100 μg/L TNF-α) and miR-126 simulant group(miR-126 simulant+100 μg/L TNF-α). Each group contented 6 duplicate samples. The expression of miR-126 was detected by quantitative r...