期刊论文

IcarisideⅡreduces myocardial ischemia-reperfusion injury through activating silent information regulator 1/acetylated-forkhead box O1pathway

中文

中华高血压杂志

1673-7245

2020

28

8

744-749

10.16439/j.cnki.1673-7245.2020.08.015

本校为第一机构

目的 探究淫羊藿次苷Ⅱ(ICSⅡ)预处理对心肌缺血再灌注损伤大鼠的保护作用,初步探究可能的作用机制.方法 将大鼠分为假手术组、模型组、淫羊藿次苷Ⅱ(ICSⅡ)组、沉默信息调控子1(SIRT1)抑制剂(Selisistat)组、ICSⅡ+Selisistat组;各组大鼠预处理后采用左冠状动脉前降支结扎法建立心肌缺血再灌注损伤模型大鼠.超声检测大鼠心室功能变化;HE染色观察大鼠心肌组织病理学变化情况;酶联免疫吸附法检测血清白细胞介素1β(IL-1β)、心肌肌钙蛋白1(cTnI)和肿瘤坏死因子αt(TNF-α)水平;Tunel染色法检测心肌组织凋亡;免疫印迹法检测心肌组织中SIRT1、乙酰化叉头蛋白O1(Ac-FOXO1)、Bcl-2相关X蛋白(Bax)、天...

Objective To observe the effects and mechanism of icarisideⅡ (ICSⅡ)on myocardial ischemia-reperfusion injury in rats. Methods The rats were divided into sham operation(Sham)group,model(Model)group, ICSⅡgroup,Selisistat[(silent information regulator 1,SIRT1)inhibitor]group,and ICSⅡ+ Selisistat group. After pretreatment,the rat model of myocardial ischemia-reperfusion injury was established by ligation of anterior descending branch of left coronary artery. The changes of ventricular function were detected by ultrasound. HE staining was used t...