期刊论文

中文

中国新药与临床杂志

1007-7669

2010

29

8

611-616

湖南省科技厅科研项目（2008SKJ16）；

本校为第一机构

目的观察艾溴利平对高糖诱导人脐静脉内皮细胞凋亡和蛋白激酶B(PKB)磷酸化的影响,并探讨其分子机制.方法应用终浓度为33.3 mmol·L-1葡萄糖诱导内皮细胞HUVEC-12细胞株高糖模型,实验分为正常对照组,高糖模型组,艾溴利平组和抑制剂组, Hoechst33258染色及Annexin V和PI双染流式细胞术检测细胞凋亡;Western blotting检测PKB总蛋白,PKB苏氨酸308(PKB-Thr308)及PKB丝氨酸473(PKB-Ser473)的磷酸化水平.结果高糖模型组内皮细胞凋亡指数(AI)增加为(14.07±s 0.25)%,高于正常对照组(P

AIM To observe the effect of ibrolipim on the apoptosis and protein kinase B （PKB） phasphorylation in cultured human umbilical vein endothelial cells （HUVECs） induced by high glucose, and to investigate its molecular mechanism. METHODS HUVEC-12 cell strain high glucose model groups were induced by 33.3 mmol·L^-1 D-glucose, and experiments were randomized into four groups： control group, high glucose model group, ibrolipim group, and inhibitor group. HUVECs apoptosis were assessed by fluorescent staining with Hoechst33258 and flow cytometr...