Conversion of Human Neuroglobin into a Multifunctional Peroxidase by Rational Design

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成果类型：

期刊论文

作者：

Chen, Shun-Fa;Liu, Xi-Chun;Xu, Jia-Kun;Li, Lianzhi*;Lang, Jia-Jia;...

通讯作者：

Lin, Ying-Wu;Li, Lianzhi

作者机构：

[Chen, Shun-Fa; Liu, Xi-Chun; Lin, Ying-Wu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Xu, Jia-Kun] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries,Minist Ag, Lab Marine Drugs & Byprod,Pilot Natl Lab Marine S, Qingdao 266071, Peoples R China.

[Wen, Ge-Bo; Lang, Jia-Jia; Lin, Ying-Wu] Univ South China, Lab Prot Struct & Funct, Med Sch, Hengyang 421001, Peoples R China.

[Li, Lianzhi] Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China.

通讯机构：

[Lin, Ying-Wu] U

[Li, Lianzhi] L

Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

Univ South China, Lab Prot Struct & Funct, Med Sch, Hengyang 421001, Peoples R China.

Liaocheng Univ, Sch Chem & Chem Engn, Liaocheng 252059, Shandong, Peoples R China.

语种：

英文

期刊：

INORGANIC CHEMISTRY

ISSN：

0020-1669

年：

2021

卷：

期：

页码：

2839-2845

DOI：

10.1021/acs.inorgchem.0c03777

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21977042]; Special Project of Major Scientific and Technological Innovation in Shandong Province [2018SDKJ0303-1]; Financial Fund of the Ministry of Agriculture and Rural Affairs, China [NFZX2018, 12200021]

机构署名：

本校为第一且通讯机构

院系归属：

化学化工学院

医学院

摘要：

Protein design has received much attention in the last decades. With an additional disulfide bond to enhance the protein stability, human A15C neuroglobin (Ngb) is an ideal protein scaffold for heme enzyme design. In this study, we rationally converted A15C Ngb into a multifunctional peroxidase by replacing the heme axial His64 with an Asp residue, where Asp64 and the native Lys67 at the heme distal site were proposed to act as an acid-base catalytic couple for H2O2 activation. Kinetic studies showed that the catalytic efficiency of A15C/H64D N...

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Conversion of Human Neuroglobin into a Multifunctional Peroxidase by Rational Design

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