期刊论文

中文

中国临床解剖学杂志

1001-165X

2012

30

2

203-208

10.13418/j.issn.1001-165x.2012.02.003

湖南省教育厅基金资助项目（10C0456）；

本校为第一机构

目的观察eNOS和iNOS在卵巢中的表达,并探讨其意义。方法采用免疫组化和图像分析系统检测大鼠生后各发育阶段卵巢中eNOS和iNOS的定位分布和表达。结果 0 d大鼠卵巢卵母细胞eNOS和iNOS均呈弱阳性反应;4 d后,eNOS和iNOS在正常卵母细胞中均呈强阳性,退化卵母细胞中反应非常弱;卵泡细胞与膜细胞中的iNOS在30 d前均呈强阳性表达,30 d后,生长卵泡中表达降低,闭锁卵泡中较正常生长卵泡表达增强,eNOS的表达稳定;240 d后卵巢内间质成分和闭锁卵泡增多,间质中的iNOS表达增强。360d后大鼠卵巢逐渐纤维化,iNOS染色强于eNOS。结论大鼠不同发育时期卵巢中,eNOS的表达相对稳定;iNOS的表达以性成熟后育龄期最低,随...

Objective To observe the expression of eNOS and iNOS in varies and investigate its significance.Methods Immunohistochemical technology and image analysis system were utilized to detect the expression of eNOS and iNOS in ovaries from postnatal rat at different developmental days.Results Positive reaction for eNOS and iNOS was weak in oocytes of rat ovaries at day 0.In ovaries from older rat than day 4,both of eNOS and iNOS immunoreactivity were intense in health oocytes and very weak in degenerative oocytes.Expression of iNOS was strong positive...