期刊论文

Changes and correlation of transforming growth factor-β1 and nitric oxide in lung tissue and plasma in pulmonary fibrosis induced by uranium ore dust in rats

中文

中国职业医学

2095-2619

2012

39

2

107-110

基金项目：国家自然科学基金资助项目（3067634）

本校为第一机构

目的 探讨铀矿尘致大鼠肺纤维化过程中,肺组织和血浆中转化生长因子-β1 (TGF-β1)、一氧化氮(NO)水平变化.方法 60只成年Wistar大鼠,随机分为铀矿尘组与对照组.采用一次性非暴露式气管灌注法,铀矿尘组一次性气管内灌注质量浓度为20 g/L铀矿粉尘悬液1 ml,对照组一次性气管内灌注生理氯化钠溶液1 ml.每组分别于染尘后第7、14、21、30及60天随机处死6只动物,取周围血及肺组织.肺组织形态学观察采用HE染色法;肺组织及血浆中TGF-β1水平的测定采用酶联免疫吸附法(ELISA法)；肺组织及血浆中NO水平的测定采用硝酸还原酶法.结果 实验观察期内铀矿尘致大鼠肺组织纤维增生病变；铀矿尘在致肺组织纤维化过...

Objective To study the changes of transforming growth factor-β1 （ TGF-β1 ） and nitric oxide （NO） contents in lung tissue and plasma in pulmonary fibrosis induced by uranium ore dust in rats. Methods 60 adult Wistar rats were randomly divided into a control group and a uranium ore dust group. Each rat in uranium ore dust group was exposed to 1 ml （20 g/L） of uranium ore dust suspension for once, while each rat in the control group was exposed to 1 ml of normal saline. 7, 14, 21,30 and 60 days after the exposure, 6 rats in each group were...