期刊论文

中文

中国职业医学

2095-2619

2010

37

1

26-28

湖南省教育厅科研基金(07C620)；

本校为第一机构

目的 观察氯化三丁基锡(Tributyltin chloride,TBTC1)对斑马鱼胚胎发育和幼鱼死亡的影响.方法 成年健康斑马鱼雌雄按1:1的比例交配产卵,分别用不同质量浓度的TBTC1对受精后4 h(Four-hour post-fertilization,4 hpf)斑马鱼进行染毒实验,统计斑马鱼胚胎受精后48 h的心率,72 h的孵出率,24、48、72、96、120 h的死亡率和畸形率.结果 与对照组相比较,随着TBTC1染毒质量浓度的增加,斑马鱼胚胎的孵化率降低、心跳减缓、畸形率和死亡率增加.当TBTC1达到80μg/L时,斑马鱼的48 hpf的心率由对照组的(182.5±3.6)次/min下降到(130.3±3.2)次/min,72 hpf的孵出率由对照组的97.46%下降到23.08%,并且120 hpf的死...

Objective To explore the effects of tributyltin chloride（TBTCl） on embryo development of zebrafish.Methods Healthy male and female fishes were selected to mate and spawn.Four-hour postfertilized embryos were exposed to either TBTCl vechicle（DMSO） or various concentrations of TBTCl（5,10,20,40,80 μg/L）.Embryonic hatching rate,heart rate and malformation rate were observed.Results With the increase of TBTCl concentrations,the hatching rate and the heart rate of embryo were reduced,while the malformation rate of embryo and the lethality of l...