Objective To evaluate the characteristic of developmental toxicity and its mechanism of bisphenol A (BPA) in vitro . Methods Thirteen day Wistar rat embryo midbrain (central nervous system, CNS) were exposed to BPA at concentrations of 0,10,20,30,40,50, or 60 mg/L in the culture medium. Results When midbrain cells were treated with BPA for 5 days, induction of cytotoxi city and inhibition of cell differentiation were observed in a concentration dependent manner. The higher level of BPA appeared to be cytotoxic to Wistar rat embryo midbrain cells in culture and reduced the number of differentia...