期刊论文

中文

中国职业医学

2095-2619

2003

30

5

13-15

10.3969/j.issn.1000-6486.2003.05.005

本校为第一机构

目的观察烹调油烟冷凝物(Cooking Oil Fume Condensates, COFC)的体外发育毒性并探讨其可能的毒性作用机制.方法采用体外全胚胎培养模型,将植入后8.5 d龄昆明种小鼠胚胎在大鼠即刻离心血清培养基中培养48 h(COFC染毒浓度分别为0、20、40、80、160、320 mg/L),观察COFC对小鼠胚胎生长发育和组织器官形态分化的影响.结果 COFC在≥80 mg/L以上剂量时能抑制胚胎生长发育和形态分化,诱发卵黄囊生长和血管分化不良,发育异常率增高;高浓度组能诱发多种畸形,主要为神经系统,心脏、肢芽等器官系统畸形.结论体外实验条件下,COFC对小鼠胚胎具有一定的胚胎毒性和致畸性.

Objective To observe the developmental toxicity and to explore the possible mechanisms of the cooking oil fume condensates (COFC) on mouse embryos in vitro . Methods The postimplantation whole embryo culture system was used. The day 8 5 isolated KM mouse embryos (2-5 somites ) at organogenetic stage were cultured for 48 hours in rat immediately centrifugal serum (ICS) with COFC ( final concentration 0,20, 40, 80, 160 and 320 mg/L ) in the presence of rat liver S 9 mix. Results COFC at high level (≥80mg/L) could inhibit the growth and blood ves...