期刊论文

中文

国际医药卫生导报

1007-1245

2011

17

18

2280-2282

10.3760/cma.j.issn.1007-1245.2011.18.029

本校为第一机构

目的 介绍一种组织芯片的手工制备技术。方法 利用改进的穿刺针、盖玻片朔料包装盒及X线废片手工制作组织芯片，进行免疫组织化学和常规HE染色。结果 阵列蜡块完整，组织蜡芯排列整齐有序、无移位，组织芯片切片染色效果满意。组织位点的形态可观测率为94.37％。结论 手工制备组织芯片方法简单易行、费用低廉；制片质量可媲美进口的组织微阵列仪或组织芯片制作机的制片质量，且易于推广。

Objective To introduce a technology of manual manufacturing tissue microarray.Methods The improved needle, coverslip plastic packaging box and X ray film were used to produce hand-made tissue microarray, and then conducted with immunohistochemistry (IHC) and HE staining.Results A complete array of wax, neat and orderly core of tissue microarray were found, without any shift,and with satisfactory staining of tissue microarray sections.Conclusion The method of hand-made preparation of tissue microarray is simple with less cost, and the quality could be matching that of the imported tissue microa...