Localization and characterization of two putative TMH family proteins in Chlamydia psittaci

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成果类型：

期刊论文

作者：

Wu, Haiying;Wang, Chuan;Jiang, Chuanhao;Xie, Yafeng;Liu, Liangzhuan;...

通讯作者：

Wu, Yimou

作者机构：

[Jiang, Chuanhao; Wu, Yimou; Song, Ying; Xie, Yafeng; Liu, Liangzhuan; Wang, Chuan; Wu, Haiying; Ma, Xiaohua] Univ South China, Coll Med, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

[Wu, Haiying] Univ South China, Affiliated Hosp 2, Dept Lab Med, Hengyang 421001, Peoples R China.

[Xie, Yafeng; Wang, Chuan] Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang 421001, Peoples R China.

[Wu, Yimou] Univ South China, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

通讯机构：

[Wu, Yimou] U

Univ South China, Inst Pathogen Biol, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang 421001, Peoples R China.

语种：

英文

关键词：

Chlamydia psittaci;Putative TMH family protein;CPSIT_0844;CPSIT_0846;Inclusion membrane protein

期刊：

Microbiological Research

ISSN：

0944-5013

年：

2016

卷：

183

页码：

19-25

DOI：

10.1016/j.micres.2015.11.005

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [81171588]; Special Foundation of Hunan Provincial Science and Technology Department [2013TT1003]; Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control Foundation [2014-5, 2012-312]; Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study [2015-351]

机构署名：

本校为第一且通讯机构

院系归属：

医学院

摘要：

Chlamydia psittaci (C. psittaci), an obligate intracellular agent of psittacosis, causes an atypical pneumonia in humans. The transmembrane head proteins (TMH) of C. psittaci, putatively belong to the Inc family and presumably play similar roles. CPSIT_0844 and CPSIT_0846 were the putative TMH proteins of C psittaci. To identify these two proteins, antisera were raised with fusion proteins which were prokaryotic expressed in Escherichia coli and purified. By immunofluorescence assay, CPSIT_0844 and CPSIT_0846 were localized in the inclusion membrane of C psittaci-infected cells. By RT-PCR and ...

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Localization and characterization of two putative TMH family proteins in Chlamydia psittaci

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