期刊论文

Cheng, J.-B.

中文

中国生物工程杂志

1671-8135

2022

42

7

1-11

10.13523/j.cb.2202052

国家自然科学基金重点项目(81930029)资助项目。

本校为第一机构

目的:阐明低氧对神经细胞在能量代谢、氧化应激、炎症等方面的影响,探讨不同类型神经细胞响应低氧刺激的敏感性,为低氧诱导的脑损伤病理机制研究提供理论基础。方法:将不同类型的神经细胞系(BV2、N9、Gl261、HT22)低氧(0.1% O_2,5% CO_2)处理0 ~24 h,用CCK8法(Cell Counting Kit-8)检测细胞增殖,发光细胞活力测定法(luminescent cell viability assay)检测细胞活力。提取各细胞总RNA及总蛋白质,通过实时荧光定量PCR (quantitative real time-PCR,RT-qPCR)、免疫印迹(Western blot)、流式细胞仪等技术检测能量代谢、炎症相关基因表达水平和氧化应激水平。结果:低氧处理后,各类神经细胞增殖明显减...

Objective: In order to illustrate the hypoxia-induced changes of neural cells in inflammatory response, oxidative stress, ad eeergy metabolism process and to compare the sezsitivity of neural cells ’ responses to hypoxia. Methods: Different types of neural cells (BV2, N9, GI261, HT22) were treated with hypoxia (0. 1% O2, 5% CO2) for0-24 hours. Cell proliferation was detected by Cell Counting Kit-8 method and cell viability was assayed by CellTiter-Glo Luminescent Cell Viability Assay. Total RNA was extracted by Trizol reagent, and the inflamma...