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Rapid Detection of Broad-Spectrum Pathogenic Bacteria Based on Highly Sensitive Proton Response of the Nucleic Acid Amplification SPQC Platform

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成果类型:
期刊论文
作者:
Liao, Yusheng;Liu, Yu;Feng, Ye;Zhen, Deshuai;He, Fengjiao
通讯作者:
He, FJ
作者机构:
[Liu, Yu; Feng, Ye; Liao, Yusheng; He, Fengjiao] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
[Zhen, Deshuai] Univ South China, Sch Publ Hlth, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China.
通讯机构:
[He, FJ ] H
Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Peoples R China.
语种:
英文
期刊:
Analytical Chemistry
ISSN:
0003-2700
年:
2024
卷:
96
期:
17
页码:
6756-6763
基金类别:
National Natural Science Foundation of China [22074031]; National Natural Science Foundation of China [2022SK2100]; Program for Science and Technology Innovation Talents of Hunan Province
机构署名:
本校为其他机构
院系归属:
公共卫生学院
摘要:
Pathogenic bacteria significantly contribute to elevated morbidity and mortality rates, highlighting the urgent need for early and precise detection. Currently, there is a paucity of effective broad-spectrum methods for detecting pathogenic bacteria. We have developed an innovative proton-responsive series piezoelectric quartz crystal (PR-SPQC) platform for the broad-spectrum identification of pathogenic bacteria. This was achieved by retrieving and aligning sequences from the NCBI GenBank database to identify and validate 16S rRNA oligonucleotide sequences that are signatures of pathogenic ba...

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