版权说明 操作指南
首页 > 成果 > 详情

A Novel Color Modulation Analysis Strategy through Tunable Multiband Laser for Nanoparticle Identification and Evaluation

认领
导出
Link by DOI
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Cao, Xuan;Lei, Gang;Feng, Jingjing;Pan, Qi;Wen, Xiaodong;He, Yan*
通讯作者:
He, Yan
作者机构:
[He, Yan; Cao, Xuan] Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
[Wen, Xiaodong; Pan, Qi; He, Yan; Lei, Gang; Feng, Jingjing] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China.
[Cao, Xuan] Univ South China, Inst Pharm & Pharmacol, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[He, Yan] Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
[He, Yan] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China.
语种:
英文
期刊:
Analytical Chemistry
ISSN:
0003-2700
年:
2018
卷:
90
期:
4
页码:
2501-2507
基金类别:
National Natural Science Foundation of China [21127009, 21425519, 21221003]; Tsinghua University Startup fund
机构署名:
本校为其他机构
院系归属:
药学与生物科学学院
摘要:
Creating color difference and improving the color resolution in digital imaging is crucial for better application of color analysis. Herein, a novel color modulation analysis strategy was developed by using a homemade tunable multiband laser illumination device, in which the portions of R, G, and B components of the illumination light are discretionarily adjustable, and hence the sample color could be visually modulated continuously in the RGB color space. Through this strategy, the color appearance of single gold nanorods (AuNRs) under dark-field microscopy was migrated from the spectrally insensitive red region to the spectrally sensitive green-yellow region. Unlike the traditional continuous-wave light source illumination, wherein the small spectral variations in the samples within a narrow spectral range are averaged by the whole spectrum of the light source, leading to little color difference, the application of sharp, multiband laser illumination could enlarge the color separation between samples, thus resulting in high spectral sensitivity in color analysis. By comparing the corresponding color evolution processes of different samples as the multiband combination of the laser illumination was changed, more efficient color separation of AuNRs was achieved. With this instrument and single [email protected] as the sulfide probe, we achieved high throughput and highly sensitive detection of sulfide at a detection limit of 0.1 nM, a more than 2 orders of magnitude improvement compared to the previous color sensing scheme. This strategy could be utilized for nanoparticle identification, evaluation, and determination in biological imaging and biochemical analysis. © 2018 American Chemical Society.

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com