Small Interfering RNA-mediated Caveolin-1 Knockout on Plasminogen Activator Inhibitor-1 Expression in Insulin-stimulated Human Vascular Endothelial Cells
This work was supported by the grants from the National Natural Science Foundation of China （30400265 and 30671047）, the Youth Foundation of Hunan Province Education Department （No. 06B079） and Traditional Chinese Medicine of Hunan （No. 204061）
机构署名：
本校为第一且通讯机构
院系归属：
医学院
药学与生物科学学院
摘要：
Using human vascular endothelial cells (ECV304) as the target, we studied the effect of caveolin (CAV)-1 in the course of insulin-stimulated expression of plasminogen activator inhibitor (PAI)-1. The appropriate single-stranded oligonucleotides representing the RNAi CAV-1 gene were analyzed by Ambion software. After annealing to generate double-stranded oligonucleotides (ds oligo), it was cloned into the pENTR/U6 entry vector containing RNA polymerase III expression element by T4 DNA ligase. The short hairpin (shRNA) sequences transferred from ...
摘要(中文)：
Using human vascular endothelial cells（ECV304）as the target,we studied the effect ofcaveolin（CAV）-1 in the course of insulin-stimulated expression of plasminogen activator inhibitor（PAl）-1.The appropriate single-stranded oligonucleotides representing the RNAi CAV- 1 gene were analyzed by Ambionsoftware.After annealing to generate double-stranded oligonucleotides（ds oligo）,it was cloned into thepENTR/U6 entry vector containing RNA polymerase Ⅲ expression element by T4 DNA ligase.The shorthairpin（shRNA）sequences transferred from the p...
