期刊论文

Gan, RL

中文

生物化学与生物物理学报

1672-9145

2003

35

10

925-929

国家自然科学基金重点资助项目 (No.3 973 0 2 0 0 ) 湖南省青年科学基金资助项目 (No.JY 0 2 13 0 40 )~~

本校为第一且通讯机构

EB病毒(EBV, Epstein-Barr virus)与人类多种肿瘤有关, 尤其是与鼻咽癌和淋巴瘤的关系密切. 为此研究了EB病毒在huPBL/SCID嵌合体小鼠体内诱发人B细胞淋巴瘤的分子特性及肿瘤发生机制. 从健康成人外周血分离出淋巴细胞, 将之移植到SCID小鼠腹腔内, 实验感染EBV, 观察肿瘤的形成; 采用单向免疫扩散法连续监测小鼠血清中人IgG的含量. 分别用PCR方法检测肿瘤组织中是否存在人Alu序列, 原位杂交检测肿瘤组织中EB病毒小RNA 分子EBER-1; 免疫组织化学方法检测人白细胞分化抗原(CD45、CD20、CD45RO、CD3), 病毒基因(LMP1、EBNA2、BZLF1)的表达, 以及细胞瘤基因蛋白(p53、C-myc、Bcl-2、Bax)在诱发肿瘤中...

To identify molecular features of neoplasms associated with EB virus, human peripheral blood lymphocytes (huPBL) were isolated from healthy volunteer donors and were transplanted intraperitoneally into SCID mice, and then huPBL/SCID mice were infected with EB virus. Serum levels of human IgG were measured by unidirectional immunodiffusion assay. Human Alu sequence and EBER-1 in tumor tissues were detected with PCR and in situ hybridization. Immunohistochemical staining was used to examine leukocyte differentiation antigens (LCA, L26, UCHL1, PS1), viral gene products (LMP1, EBNA2, BZLF1) and ce...