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The Insertion Mechanism of a Living Cell Determined by the Stress Segmentation Effect of the Cell Membrane during the Tip–Cell Interaction

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成果类型:
期刊论文
作者:
Fan, Na*;Jiang, Hai;Ye, Zhiyi;Wu, Guiyong;Kang, Yuejun;...
通讯作者:
Fan, Na;Peng, Bei;Wang, Guixue
作者机构:
[Peng, Bei; Wang, Qun; Fan, Na; Fan, N; Peng, B; Jiang, Hai; Wu, Guiyong; Zhang, Guocheng] Univ Elect Sci & Technol China, Sch Mech & Elect Engn, Chengdu 611731, Sichuan, Peoples R China.
[Wang, Guixue; Ye, Zhiyi; Ran, Xiaolin] Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Coll Bioengn, Chongqing 400044, Peoples R China.
[Kang, Yuejun] Southwest Univ, Inst Clean Energy & Adv Mat, Chongqing 400715, Peoples R China.
[Guo, Jian] Univ South China, Sch Mech Engn, Hengyang 421001, Hunan, Peoples R China.
通讯机构:
[Fan, N; Peng, B] U
[Wang, Guixue] C
Univ Elect Sci & Technol China, Sch Mech & Elect Engn, Chengdu 611731, Sichuan, Peoples R China.
Chongqing Univ, Key Lab Biorheol Sci & Technol, Minist Educ, Coll Bioengn, Chongqing 400044, Peoples R China.
语种:
英文
关键词:
atomic force microscopy;cell membranes;insertion mechanism;stress concentration;stress segmentation
期刊:
Small
ISSN:
1613-6810
年:
2018
卷:
14
期:
22
页码:
e1703868
基金类别:
N.F. and H.J. contributed equally to this work. N.F., H.J., Z.Y., Q.W., G.W., and B.P. proposed the concept. N.F. performed all the experiments. N.F. and G.W. performed the calculations and simulations. H.J. and N.F. analyzed the data and wrote the manuscript. All the authors discussed the results and participated in revising of the manuscript. The authors would like to acknowledge the partial supports provided by the National Natural Science Foundation of China (Grant Nos. 31771078, 51575090, and 11172341), Project of International Science and Technology Cooperation and Exchange of Sichuan (Grant No. 2017HH0072), Fundamental Research Funds for the Central Universities (Grant Nos. ZYGX2014Z004, ZYGX2016KYQD118, and ZYGX2015J084), and the National Youth Top-Notch Talent Support Program.
机构署名:
本校为其他机构
院系归属:
机械工程学院
摘要:
Atomic force microscopy probes are proved to be powerful tools to measure and manipulate the individual cell, providing potential applications for the controlled drug/protein delivery. However, the measured insertion efficiency varies dramatically from 20 to 80%, in some cases, the nanotip can never penetrate the cell membrane no matter how much force is applied to it. Thus, the insertion mechanism of a living cell during the tip–cell interaction must be thoroughly investigated before this technology comes into practical applications. In this ...

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