期刊论文

中文

中国实验动物学报

1005-4847

2015

23

1

63-68

10.3969/j.issn.1005-4847.2015.01.012

湖南省科技厅重点科研项目（2012TTl001） 湖南省与衡阳市联合基金项目（13JJ8012） 湖南省科技厅项目（2013TT2006）.

本校为第一机构

目的观察高糖高脂联合低剂量链脲佐菌素(streptozotocin,STZ)对版纳微型猪糖、脂代谢紊乱、肝组织病理改变及其蛋白激酶B(protein kinase B,PKB)磷酸化的影响,并探讨其机制。方法高糖高脂联合低剂量STZ诱导云南版纳微型猪2型糖尿病模型,每月末测定血糖、血脂、胰岛素,HE、PAS和苏丹Ⅳ染色观察肝脏显微结构。12个月末处死动物,real time-PCR和Western blotting检测肝组织PKB mRNA和总蛋白表达及PKB丝氨酸473(PKB-Ser~(473))的磷酸化水平。结果喂养12月后,与正常对照组比较,模型组显示高血糖、血脂障碍及胰岛素缺乏(P

Objective To observe the effect of high sucrose/high fat diet and streptozotocin （STZ） on glucose and lipid metabolism, liver histology and protein kinase B （PKB） phasphorylation in Chinese Banna miniature pigs, and to in- vestigate its molecular mechanism. Methods Eight healthy 2-month old male Banna minipigs were randomly divided into control and diabetes groups, 4 pigs in each group. Diabetic minipigs were induced by high sucrose/high fat diet and STZ and the levels of fasting blood glucose, insulin, total cholesterol, triglycerides were...