期刊论文

中文

中国实验动物学报

1005-4847

2008

16

1

14-18

10.3969/j.issn.1005-4847.2008.01.004

湖南省自然科学基金（06JJ50051） 湖南省教育厅科学研究项目（06C693）.

本校为第一机构

目的克隆SD大鼠SDF-1α基因，并进行生物信息学分析。方法采用RT-PCR扩增SDF-1α基因，对扩增产物进行序列的测定，利用Internet和GenBank数据库对测序结果正确的SDF-1α基因进行生物信息学分析。结果克隆了SDF-1α基因，测序结果正确；生物信息学分析表明，SDF-1α基因开放读码为270bp，编码89个氨基酸；同源性分析表明，与人、猫、狗的SDF-1α基因同源性最大。多种组织的半定量RT-PCR研究表明，该基因在心脏、肝脏、肺、脑、肾脏、脾脏、胰脏均有表达，其中脾脏、胰脏表达较高。结论成功克隆SDF-1α基因，对其功能进行了初步预测，为进一步的功能研究打下基础。

Objective To amplify and clone SDF-1α gene and to predict its function by bioinformatic analysis. Methods The SDF-1α was amplified by RT-PCR, then identified by sequencing. Function of SDF-1α was predicted by bioinformatic analysis with Intemet and GenBank databse. Results SDF-1α was amplified and sequenced correctly. The SDF-1α contained a single open reading frame （ORF） of 270 base pairs with the potential to encode a protain of 89 and had the highest similarity with human, cat and dog. Semi-quantitative RT-PCR analysis of mRNA from di...