期刊论文

中文

中国实验动物学报

1005-4847

2003

11

2

103-106

10.3969/j.issn.1005-4847.2003.02.010

湖南省科技厅科研项目资助 （课题号 :0 0JZN2 0 3 2 ）；

本校为第一机构

目的建立饮食诱导新西兰兔糖尿病模型,探讨脂肪毒性和葡萄糖毒性在2型糖尿病发病中的意义.方法将雄性新西兰兔30只按血糖血脂浓度随机分为2组,15只饲以基础饲料作为正常对照组(C组);15只饲以高糖高脂饲料作为实验组(DD组),共观察32周,每4周从禁食过夜的兔耳静脉抽取血样,测定血清中血糖、胰岛素、甘油三酯.于32周时,全部处死动物,取动物胰腺.取部分胰腺用10%中性甲醛液固定,常规石蜡包埋切片,H、E染色,光学显微镜下观察;另一部分胰腺用2.5%戊二醛固定,常规电镜样品制备,透射电镜下观察并照相.结果实验组4周后即出现高血糖、高甘油三酯,并随着喂养时间延长,而有所升高,基础饲料组血糖、甘油三酯未...

Objective To induce diabetes in New Zealand white rabbits by high fat/high sucrose feeding and observe the role of lipotoxicity and glucotoxicity in the development of diabetes. Methods Male New Zealand white rabbits were divided into two groups:normal fodder group (n=15) and high sugar and high fat fodder group (n=15, with 10% lard, 37% white cane sugar and 53% normal fodder), and observed for 32 weeks. Once a month after a overnight fasting the blood samples of the rabbits were tested for the levels of blood glucose, insulin, and triglyceride...