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Assessment of recombinant antigens Tp0100 and Tp1016 of Treponema pallidum for serological diagnosis of syphilis

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成果类型:
期刊论文
作者:
Chen, Dejun;Wang, Siqian;He, Yuxing;Fu, Yue;Zhao, Feijun;...
通讯作者:
Longgu Cao<&wdkj&>Tiebing Zeng
作者机构:
[Fu, Yue; Wu, Yimou; Zhao, Feijun; Chen, Dejun; He, Yuxing; Zeng, Tiebing] Univ South China, Hengyang Med Sch, Inst Pathogen Biol, Hengyang, Peoples R China.
[Fu, Yue; Wu, Yimou; Zhao, Feijun; Chen, Dejun; He, Yuxing; Zeng, Tiebing] Univ South China, Hengyang Med Sch, Hunan Prov Key Lab Special Pathogens Prevent & Co, Hengyang, Peoples R China.
[Fu, Yue; Wu, Yimou; Zhao, Feijun; Chen, Dejun; He, Yuxing; Zeng, Tiebing] Univ South China, Hunan Prov Cooperat Innovat Ctr Mol Target New Dr, Hengyang, Peoples R China.
[Wang, Siqian] First Pepoles Hosp Changde City, Clin Lab, Changde, Peoples R China.
[Zhou, Xiuping] Changsha Hlth Vocat Coll, Dept Lab Med, Changsha, Peoples R China.
通讯机构:
[Longgu Cao] C
[Tiebing Zeng] I
Institution of Pathogenic Biology and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control, Hengyang Medical School, University of South China, Hengyang, China<&wdkj&>Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, University of South China, Hengyang, China<&wdkj&>College of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou, China
语种:
英文
关键词:
bacterial antigen;immunoglobulin G antibody;Tp0100 antigen;Tp1016 antigen;unclassified drug;bacterial antigen;bacterium antibody;recombinant protein;Article;chemiluminescence immunoassay;controlled study;diagnostic test accuracy study;enzyme linked immunosorbent assay;hepatitis B;human;leptospirosis;light initiated chemiluminescent assay;Lyme disease;nonhuman;polyacrylamide gel electrophoresis;polymerase chain reaction;rheumatoid arthritis;sensitivity and specificity;serodiagnosis;syphilis;systemic lupus erythematosus;Treponema pallidum;tuberculosis;Western blotting;genetics;serology;syphilis serology;Antibodies, Bacterial;Antigens, Bacterial;Enzyme-Linked Immunosorbent Assay;Humans;Recombinant Proteins;Sensitivity and Specificity;Serologic Tests;Syphilis;Syphilis Serodiagnosis;Treponema pallidum
期刊:
Journal of Clinical Laboratory Analysis
ISSN:
0887-8013
年:
2022
卷:
36
期:
9
页码:
e24635
基金类别:
This work was supported by the National Natural Science Foundation of China (No. 81273322), Major Scientific and Technological Projects for Collaborative Prevention and Control of Birth Defects in Hunan Province (No. 2019SK1010), Scientific Research Project of Hunan Provincial Department of Education (No. 21C1650), Scientific Research Fund of Hunan Provincial Education Department (No. 20A424, 20C1732), Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study (No. 2015‐351), and Hunan Provincial Key Laboratory for Special Pathogens Prevention and Control Foundation (No. 2014‐5) and Research Learning and Innovative Experiment Project for College Students in Hunan Province (No. S201910555134, S202010555056, S202110555306).
机构署名:
本校为第一机构
院系归属:
医学院
药学与生物科学学院
摘要:
Objective: To discover novel serodiagnostic candidates for the serological diagnosis of syphilis. Methods: Two recombinant Treponema pallidum proteins Tp0100 and Tp1016 were expressed, purified, and identified by Western Blotting. A total of 600 clinical serum samples were tested with the Tp0100-based ELISA, the Tp1016-based ELISA, and the commercial LICA Syphilis TP kit (ChIVD, Beijing, China). The sensitivities were determined by testing 340 samples from individuals with clinically diagnosed primary, secondary, latent, and tertiary syphilis. ...

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