Increased trans-endothelial cells permeability induced by oxidized lipoprotein(a) relates to reactive oxygen species generation and desmogleins expression
Aim: we aim at measurement enhanced effect of oxidized lipoprotien(a) [oxLp(a)] on permeability of monolayer endothelial cells and relationship with reactive oxygen spe-cies(ROS) generation and desmogleins(DSGs) expression. Methods and Results : Trans-endothelial permeability was assayed by transwell and reactive oxygen species(ROS) was determined by DCFH-DA staining. RT-PCR was carried out to determine DSG1 and DSC2 expression in mRNA, respectively. Trans-endothelial permeability was enhanced by oxLP(a) dose and time dependently. The most mark...
