期刊论文

Construction of human ROR alpha miRNA eukaryotic expression vector and its effects on proliferation in gastric cancer cells

中文

中国现代医药杂志

1672-9463

2011

13

2

1-4

10.3969/j.issn.1672-9463.2011.02.001

国家自然科学基金资助项目（编号:81071966）； 湖南省高校创新平台开放基金项目（编号:09K074）； 湖南省高校重点实验室(肿瘤细胞与分子病理学)资助项目；

本校为第一机构

目的 构建RORα(Retinoid acid receptor related orphan receptor α,维甲酸相关孤核受体α)miRNA真核表达载体,分析其对人胃癌MGC803细胞RORα蛋白表达及细胞增殖的影响.方法 构建针对设计RORα miRNA表达的3对miRNA干扰序列,克隆入pcDNATM6.2-GW/EmGFP miRNA真核表达载体,在测序鉴定无误后将含RORα miRNA转染人胃癌MGC803细胞,用Western blo检测MGC803细胞转染前后RORα蛋白的表达水平变化.MTT法检测对转染MGC803细胞增殖的影响.结果 Western blot结果证实,MGC803细胞转染RORα miRNA后24h RORα蛋白表达水平明显低于转染阴性组(P＜0.05).MTT结果显示,转染RORα miRNA后细胞增殖率(光吸收值...

Objective To construct a ROR alpha miRNA expression vector,and evaluate its effects on ROR alpha protein expressions and proliferation in MGC803 gastric cancer cells in vitro. Methods Three pairs of RNA interference sequences of ROR alpha targetting RORα miRNA were cloned into the eukaryotic expression vector pcDNATM6.2-GW/EmGFP.After sequencing,the vector was then transfected into MGC803 cells. The change of ROR alpha protein expression in transfected cells were demonstrated by Western blot.The impact on proliferation in transfected MGC803 ce...