期刊论文

Effects of inducing apoptosis of the acetoacetate extract of Vitex negundo seed enhances cisplatin on the human ovarian carcinoma cells COC1

中文

中国现代医药杂志

1672-9463

2009

11

5

5-8

10.3969/j.issn.1672-9463.2009.05.002

本校为第一机构

目的 观察黄荆子乙酸乙酯提取物(the acetoacetate extract of Vitex negundo seed,Evn-50)和顺铂(cisplatin,DDP)在体外对人卵巢癌细胞株COC1凋亡的影响,探讨Evn-50提高COC1细胞株对顺铂化疗敏感性的机制.方法 Evn-50,DDP以及两药联合分别处理人卵巢癌COC1细胞株,台盼蓝染色计数法检测药物对细胞生长的抑制率,并用流式细胞术、AO/CB荧光双染色法测Evn-50,DDP以及两药联合对细胞凋亡的影响.结果 细胞抑制率往联用组中随Evn-50剂量的增加而增加,且明显高于同剂量单用组(P

Objective To explore the effects of the acetoacetate extract of Vitex negundo seed （Evn-50） and cisplatin （DDP）on the inducing apoptosis of ovarian carcinoma cell line COC1 in vitro and the potential mechanism of increased response rate of DDP induced by Evn-50. Methods Human ovarian carcinoma cell line,COCl,was respectively treated by Evn-50, DDP and Evn-50 combined with DDP. Growth inhibition was measured by trypan blue exclusion test.The arrest of cell cycle and the influence induce human ovarian carcinoma cell line COC1 apoptosis after ...