期刊论文

中文

应用化学

1000-0518

2009

26

3

277-281

10.3969/j.issn.1000-0518.2009.03.007

2006AA09Z422:国家"八六三"科技攻关计划 20072058:国家自然科学基金 021732:广东省自然科学基金重点项目 5-2007-XQD-002:南华大学博士启动基金

本校为第一机构

通过人工发酵培养,利用层析技术,从南海红树林内源真菌#2240的培养液中分离得到3个蒽醌类次级代谢产物,它们分别是:(+)-3,3',7,7',8,8'-六羟基-5,5'-二甲基连二蒽醌(化合物1),1,4,5-三羟基-7-甲基蒽醌(化合物2)和1,6,8-三羟基-3-甲基蒽醌(化合物3),其中化合物1为新化合物. 它们的结构通过谱图分析(包括~1H、~(13)C NMR、DEPT、HMQC、HMBC、HREIMS、UV-Vis及IR等)和相关文献得以确定. 初步药理实验表明,化合物1对DNA拓扑异构酶(hTopo Ⅰ)具有强抑制活性,最低抑制浓度2.35×10~(-4) mol/L,结果远低于阳性对照物喜树碱之值1.00×10~(-3) mol/L.

Three anthraquinone secondary metabolites, （ ＋ ） -3,3＇, 7,7＇, 8,8＇-hexahydroxy-5, 5＇-dimethyl- bianthraquinone （ compound 1 ）, 1,4, 5-thihydroxy-7-methylanthraquinone （ compound 2 ）, and 1, 6, 8-thihydroxy-3-methylanthraquinone （compound 3 ） , were isolated from the culture media of mangrove endophytic fungus #2240 collected from a mangrove Castaniopsic Fissa from South China Sea coast through chromatographic separation. Compound 1 was found for the first time. Their structures were elucidated based on related literature and spectr...