期刊论文

Biological Effects and RAPD Analysis of ~(60)Co-γ-radiation on Sasapygmaea

中文

世界科技研究与发展

1006-6055

2016

38

6

1280-1286

10.16507/j.issn.1006-6055.2016.06.031

北京师范大学本科生科研训练与创新创业基金

本校为其他机构

用不同剂量(5、10、15、20和25 Gy)的~(60)Co-γ射线对翠竹幼苗进行辐照处理,结果显示,辐照导致翠竹矮化,植株的株高、节间长度、叶长及叶宽等性状较对照组显著变短;并且10 Gy辐照导致翠竹部分叶片白化。基因组RAPD分子标记检测显示,辐照组扩增的DNA片段图谱发生显著变化,表明经辐照处理的翠竹基因组发生了变异。其中,5 Gy辐照实验组的变异程度最小,25 Gy辐照实验组的变异程度最大。研究表明,~(60)Co-γ射线辐照可以引起翠竹植株矮化,并导致基因组变异。本研究为进一步进行翠竹辐照诱变育种打下了基础。

Various radiation dosages(5 Gy、10 Gy、15 Gy、20 Gy and 25 Gy) of ~(60)Co-γ-radiation are used to treat the seedlings of Sasapygmaea. The results show that irradiation can induce dwarf effect, and the plant height, internode length, leaf length and width of irradiation group are significantly shorter than the control group. The leaves of Sasapygmaea in 10 Gy radiation dosage group show albinism. By genomic RAPD assay, the amplification of DNA bands show significantly change, which suggests the genome of Sasapygmaea underwent mutation after irr...