期刊论文

Dihydromyricin inhibited hepatic lipid deposition induced by high-fat diet in obese mice by activating SIRT1-AMPK pathway

中文

中国药理学通报

1001-1978

2021

37

01

107-113

10.3969/j.issn.1001-1978.2021.01.017

湖南省自然科学基金资助项目（No 2018JJ2347）,湖南省研究生科研创新项目（No CX20190754）； 南华大学大学生研究性学习和创新性实验计划项目（No 2018XJXZ16）；

本校为第一机构

目的探究二氢杨梅素（dihydromyricetin,DHM）对高脂饮食诱导的肥胖小鼠肝脏脂质蓄积的作用及其机制。方法 C57BL/6J小鼠60只,随机分为6组（n=10）:（1）ND组:正常饲料;（2）ND+L-DHM组:正常饲料+低剂量DHM(125 mg·kg-1·d-1);（3）ND+H-DHM组:正常饲料+高剂量DHM(250 mg·kg-1·d-1);（4）HFD组:高脂饲料;（5）HFD+L-DHM组:高脂饲料+低剂量DHM;（6）HFD+H-DHM组:高脂饲料+高剂量DHM。记录小鼠体重;16周后,测空腹血脂;计算体脂重量;肝脏HE和油红O染色;荧光定量PCR和Western blot检测肝脏SIRT1、AMPK、ACC、FAS、SREBP-1和PPARα、CPT1的表达。结果与ND组相比,HFD组小鼠体重、体脂、血清TG、TC、...

Aim To investigate the effect of dihydromyricetin (DHM) on lipid accumulation in liver of obese mice induced by high fat diet and its mechanism. Methods Sixty C57BL/6J mices were randomly divided into six groups (n = 10); (1)ND group; normal diet, (2)ND + L-DHM group; normal diet and treatment with low-dose DHM (125 mg • kg-1 • d - 1 ), (3)ND + H-DHM group; normal diet and treatment with high-dose DHM (250 mg • kg-1 • d - 1 ), (4) HFD group; high-fat diet, and (5)HFD + L-DHM group; high-fat diet and treatment with low-dose DHM, (6) HFD + H-...