期刊论文

The protective effect of 1,25-dihydroxyvitamin-D_3 on hydrogen peroxide-induced MIN6 cell apoptosis

中文

中国药理学通报

1001-1978

2020

36

2

198-203

10.3969/j.issn.1001-1978.2020.02.010

湖南省自然科学基金资助项目(No 2016JJ2113) 湖南省教育厅科学研究重点项目(No 16A186) 湖南省大学生研究性学习和创新性实验计划项目资助(No S201910555141)。

本校为第一机构

目的探讨维生素D(vitamin D,VitD)对过氧化氢(hydrogen peroxide, H_2O_2 )诱导小鼠胰岛β细胞株MIN6细胞凋亡的保护作用及机制。方法VitD预处理后,用H_2O_2处理MIN6细胞,分别运用CCK-8法、Hoechst 33258荧光染色法、流式细胞术检测MIN6细胞增殖、形态及凋亡百分率。Western blot检测增殖与凋亡相关基因Bax、Bcl-2、Caspase-3以及Cleaved caspase-3的表达。结果H_2O_2呈时间和剂量依赖性抑制MIN6细胞增殖,诱导细胞凋亡,降低Bcl-2表达、增加Bax及Cleaved caspase-3表达,Bcl-2/Bax比值降低,Cleaved caspase-3/caspase-3比值增加;当用VitD预处理后,Bcl-2表达增加,Bax、Cleaved caspase-3表达降低,B...

Aim To investigate the role and mechanism of VitD in protecting apoptosis of mouse islet (3 cell line MIN6 cells induced by H202. Methods MIN6 cells were treated with H202 after the pretreat-ment of VitD. The proliferation, morphological changes and apoptotic percentage of MIN6 cells were determined by CCK-8, Hoechst 33258 fluorescence staining and flow cytometry respectively. The expressions of ap-optosis-related genes Bel-2, Bax, Caspase-3 and Cleaved caspase-3 were detected by Western blot. Results H202 inhibited proliferation and induced ap...