期刊论文

Interacting of receptor component protein and G protein in static pressure-induced proliferation of VSMC

中文

中国药理学通报

1001-1978

2017

33

8

1170-1175

10.3969/j.issn.1001-1978.2017.08.025

No30572192:国家自然科学基金 81173060:国家自然科学基金 No2011XCX13:南华大学研究生科研创新项目 0223-0002-00031:南华大学研究生科研创新项目

本校为其他机构

目的 探讨受体组分蛋白(RCP)在静压力促血管平滑肌细胞(VSMC)增殖信号跨膜转导中的作用.方法 以鼠源性A10血管平滑肌细胞(A10VSMC)作为研究对象,用静压力处理细胞,噻唑蓝比色法(MTT)检测细胞的活性;Western blot 检测增殖细胞核抗原(PCNA)、RCP和G蛋白的表达;RT-PCR检测RCP mRNA表达水平;免疫共沉淀检测G蛋白和RCP间相互作用.结果 静压力呈压力和时间依赖性地增加A10VSMC活性、PCNA蛋白表达和RCP表达,并在120 mmHg静压力和6 h达到最大.静压力在增加细胞 p-Akt表达水平(P

Aim: To explore the effect of receptor component protein (RCP) in the signal transduction of vascular smooth muscle cell (VSMC) proliferation induced by static pressure. Methods: The mouse-derived vascular smooth muscle cell line (A10VSMC) was employed in the experiment. Cells were exposed to static pressure, and MTT assay was used to detect the cell viability. Western blot was used to determine the expressions of PCNA, RCP and p-Akt, RCP mRNA was tested by RT-PCR, and co-immunoprecipitation was used to test the interaction between RCP and G pr...