期刊论文

中文

中国药理学通报

1001-1978

2015

31

9

1292-1298

10.3969/j.issn.1001-1978.2015.09.022

湖南省十二五重点学科建设项目 湖南省中医药管理局重点项目资助（No 201314）

本校为第一机构

目的：探讨染料木素是否通过下调 p22phox、Nox4的表达，抑制活性氧的生成而拮抗对氧磷损伤的血管内皮功能。方法取♂ SD 大鼠胸主动脉。①溶媒对照组：用0.1％的二甲基亚砜（dimethyl sulfoxide，DMSO）处理大鼠胸主动脉30 min；②染料木素（genistein，GST）处理组：GST （100μmol · L －1）处理大鼠胸主动脉30 min；③对氧磷（paraoxon，PO）处理组：PO（40.5μmol·L －1）处理大鼠胸主动脉30 min；④ PO ＋GST 处理组：PO（40.5μmol·L －1）＋GST（100μmol·L －1）处理大鼠胸主动脉30 min。RT-PCR法检测各组 p22phox、Nox4 mRNA 表达的变化；Western blot观察 p22phox 和 Nox4蛋白表...

Aim To investigate whether genistein protects paraoxon-induced vascular endothelial dysfunction through down-regulating p22phox and Nox4 expressions as well as inhibiting the generation of ROS. Methods In this study, thoracic aortas were isolated from the male Sprague-Dawley(SD) rats and were divided into the following groups: (1) control group, the thoracic aortas were incubated with dimethyl sulfoxide (DMSO, 0.1 %) for 30 min; (2) genistein group, the thoracic aortas were incubated with genistein (100 μmol • L-1) for 30 min; (3) paraoxon gr...