期刊论文

中文

中国药理学通报

1001-1978

2013

29

3

425-431

10.3969/j.issn.1001-1978.2013.03.028

湖南省教育厅重点项目（No 10A107） 湖南省自然科学基金（No 08JJ5003）

本校为第一机构

目的观察白藜芦醇(Res)对博莱霉素所致大鼠肺纤维化的影响,并探讨其是否通过转化生长因子-β1(TGF-β1)/含I型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(ADAMTS-1)信号通路起作用。方法90只SD大鼠随机分为对照组(n=10)、模型组(n=40)和Res组(n=40),后两组经气管内注入博莱霉素建立大鼠肺纤维化模型,d 2起Res组大鼠予100 mg·kg~(-1) Res灌胃,其余两组相同条件下给予生理盐水,模型组和Res组分别于d 7、d 14、d 28、d 56随机处死10只大鼠,而对照组于d 56全部处死作为总体对照,取出肺组织行HE染色和Masson染色,用荧光实时定量PCR和Western blot分别检测肺组织中TGF-β1、ADAMTS-1、Ⅰ型胶原(ColⅠ...

Aim: To observe the influence of resveratrol (Res) on bleomycin-induced pulmonary fibrosis in rats, and explore whether the effect is mediated by transforming growth factor-β1 (TGF-β1) / a disintegrin-like and metalloproteinase with thrombospondin type 1 motif (ADAMTS-1) signaling pathway. Methods: Ninety SD rats were randomly divided into control group (n = 10), model group (n = 40) and Res group(n = 40). Rats in the latter 2 groups were intratracheally administered with bleomycin to produce pulmonary fibrosis. From the second day, rats in i...