期刊论文

中文

中国药理学通报

1001-1978

2013

29

12

1660-1663

10.3969/j.issn.1001-1978.2013.12.008

基金项日：国家自然科学基金资助项目（No81200590） 湖南省教育厅资助科研项目（No11C1093,11C1095）

本校为第一机构

目的研究Neu-p11预处理对大鼠心肌缺血/再灌注损伤细胞凋亡的影响。方法30只♂ SD大鼠随机分为3组:假手术组，缺血/再灌注损伤组和Neu-p11预处理组。测定血清中乳酸脱氢酶(LDH) 、肌酸激酶(CK)的活性及心肌丙二醛(MDA)的含量，TUNEL法检测心肌细胞凋亡指数，免疫印迹法检测Bcl-2、Bax蛋白的表达。结果Neu-p11预处理可明显改善缺血/再灌注损伤大鼠的心功能损害，减少血清中LDH、CK 的释放和心肌组织中MDA的产生，可使心肌细胞凋亡率减少，心肌Bcl-2蛋白表达上调，Bax 蛋白表达下调。结论Neu-p11对心肌缺血/再灌注损伤具有保护作用，其机制可能与抑制细胞凋亡有关。

Aim: To study the effect of Neu-p11 on myocardial ischemia-reperfusion injury in rats. Methods: Thirty male Wistar rats were randomly divided into three groups: sham group,I/R group and Neu-p11 group. LDH and CK activity in serum and MDA content in myocardial tissue were measured. Cell apoptosis was evaluated by TUNEL assay. Bcl-2 and Bax protein expressions were detected by Western blot. Results: Neu-p11 significantly decreased the level of LDH, CK and MDA content. The myocardial apoptotic index in Neu-p11 group was markedly lower than that in I/R group. And Western blot analysis revealed tha...