期刊论文

Yan, L.-M.

中文

中国药理学通报

1001-1978

2010

26

4

483-487

30670834:国家自然科学基金 30871186:国家自然科学基金

本校为第一且通讯机构

目的 探讨microRNAs对脂肪细胞分化过程的调节作用分子机制及其对脂肪特异性基因--脂肪型脂肪酸结合蛋白(fatty acid binding protein, FABP4)表达的影响.方法 用microRNAs微阵列分析法筛选和鉴定3T3-L1脂肪细胞分化相关性microRNAs,构建脂肪细胞分化相关性micro-RNAs的高表达质粒,通过脂质体介导转染3T3-L1前体脂肪细胞,观察脂肪相关性microRNAs对脂肪细胞分化过程的影响,Western blot 和RT-PCR分别检测FABP4蛋白及其mRNA在脂肪细胞分化过程中的表达变化.结果 3T3-L1脂肪细胞分化过程中microRNA表达谱发生明显改变,其中35个microRNAs下调,miR-24最明显;17个microRNAs上调,miR-21最明显;用不同脂...

Aim: To explore the effect of microRNAs on the differentiation of 3T3-L1 adipocytes and the expression of adipo-related gene-fatty acid binding protein during the adipocyte differentiation. Methods adiporelated microRNAs during 3T3-L1 adipocyte differentiation were screened and identified by micorRNA microarray. Constructed high-expression plasmids of the adipo-related microRNAs, were transfected into the 3T3-L1 preadipocytes by lipofectamine. While the effect of adipo-related microRNAs on the course of 3T3-L1 adipocyte differentiation was observed, the protein and mRNA expression level of fat...