期刊论文

Pan, W.-N.

中文

中国药理学通报

1001-1978

2008

24

2

214-218

10.3321/j.issn:1001-1978.2008.02.018

国家自然科学基金资助项目（No30371645） 湖南省教育厅优秀青年基金资助项目（No038036）

本校为第一且通讯机构

目的研究G蛋白偶联受体APJ（血管紧张素受体样受体或称血管紧张素受体AT．相关的受体蛋白，putative receptor protein related to the angiotensinreceptor AT_1）的内源性配体apelin-13通过PKC—ERK1／2-Cyclins信号通路促进大鼠血管平滑肌细胞（vascular smooth muscle cells，VSMCs）增殖的影响。方法培养SD大鼠胸主动脉VSMCs，Western blot检测p-ERK1／2、ERK1／2、细胞周期蛋白CyclinDl和CyclinE的表达，四噻唑蓝比色法观察PKC阻断剂GF109203X对apelin-13促大鼠VSMCs增殖的影响。结果Apelin-13剂量依赖性和时间依赖性地促进大鼠VSMCsp-ERK1／2表达增加，对ERK1／2表达没有明显影响，GF109...

Aim: To investigate the effects of cell proliferation induced by apelin-13 via PKC-ERK1/2-Cyclins signaling pathway in rat vascular smooth muscle cells (VSMCs). Further to find new signaling pathway of apelin-APJ. Methods: VSMCs were prepared from male Sprague-Dawley rats'thoracic aortas by the primary-explant method. Expression of p-ERK1/2, ERK1/2, CyclinD1 and CyclinE was detected by Western blot. The blockade effects of GF109203X were measured by MTT assay. Results: Apelin-13 promoted the concentration-dependent and time-dependent expression...